Green fluorescent protein (GFP) has gained widespread use as a tool to visualize spatial and temporal patterns of gene expression in vivo. However, it is not generally accepted that GFP can also be used as a quantitative reporter of gene expression.

GFP is great as a reporter protein because it has no effect on the host when introduced to the host's cellular environment. Due to this ability, no external visualization stain, ATP, or cofactors are needed.

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is rapidly becoming an important reporter molecule for monitoring gene expression and protein localization in vivo, in situ and in real time.

This protocol describes the process of inserting a green fluorescent protein (GFP) reporter into the IAV genome using the established reverse genetics system.

The use of green fluorescent protein (GFP) as a reporter for protein localization in Escherichia coli was explored by creating gene fusions between malE (encoding maltose-binding protein [MBP]) and a variant of gfp optimized for fluorescence in bacteria (GFPuv). These constructs encode hybrid proteins composed of GFP fused to the carboxy ...

Transcription Reporter - Here, the GFP is placed under the control of a promoter of interest. As such, it helps monitor gene expression from the promoter in a given type of cell.

The DR-GFP reporter consists of two tandem, inactive copies of the GFP gene, which can be integrated into the cellular genome or expressed transiently. The first GFP copy (SceGFP) has a promoter and contains the I-SceI restriction site with an in-frame stop codon and the second copy (iGFP) harbours truncations at both ends [ 8 , 9 ].

By fusing the GFP gene with another gene, scientists can use GFP as reporter gene in biological studies, signaling gene expression or the location of a protein in a cell. We have used GFP to signal the expression of particular genes during microbial biofilm development.

Dec 16, 2008 · Our findings demonstrate that iLOV represents a new class of genetically encoded FP that outperforms GFP as a reporter for plant virus movement and confers improved viral protein functionality.

Sep 4, 2020 · The green fluorescent protein (GFP) is a powerful reporter protein that allows labeling of specific proteins or entire cells.