Frontiers23d
Research advances in replication-deficient viral vector vaccines
In normal cells, one or more steps of viral replication are blocked, resulting in viral gene expression within ... coexpressed the G protein of IHNV and the VP2 protein of IPNV by inserting them into ...
Frontiers26d
Chromatin structure and gene transcription of recombinant p53 adenovirus vector within host
it does not alter the expression of associated host genes or disrupt the TADs and A/B compartment structures of the host genome. The therapeutic effects of Ad-p53 are likely primarily mediated by the ...
GEN27d
New Technologies Make Plant Cell-Based Protein Platforms Competitive
making plant cell-based bioproduction a transformative platform for producing high-value recombinant proteins. “Implementing these strategies would significantly improve the efficiency of plant ...
eLife29d
Inhibiting NINJ1-dependent plasma membrane rupture protects against inflammasomeinduced blood coagulation and inflammation
Recombinant proteins were expressed using LPS free E. coli strain ClearColi BL21(DE3) (Lucigen Corporation, Cat#60810) at 37 °C for 4 hours with 500 μM IPTG after OD600 reached 0.6-0.8. Bacteria were ...