Transgenic animals continue to play an essential role in many aspects of zebrafish research, including the development of disease models. The most widely used system for zebrafish transgenesis is the ...

Open-Source package for segmentation and analysis of gel electrophoresis images using machine learning. Both a python framework for training/running models and a QuPath GUI extension are available.

School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P.R. China School of Chemical & Material Engineering, Jiangnan University, Wuxi 214122, P.R. China ...

Scientists commonly do this using gel electrophoresis analysis, which can determine plasmid length and conformation based on how DNA migrates within a gel. The Agilent Fragment Analyzer system offers ...

Here we introduce a process called Improved Single-cell Genome Amplification (iSGA), in which the whole single-cell sequencing cycle is completed in a high-efficient and high-coverage manner, through ...

The amount of DNA used for lipofection was 1 μg per well. Transfection efficiency was always higher than 90% as determined by fluorescent microscopy or flow cytometry, following the delivery of a ...

During virus infection detection of nucleic acids is crucial for the inflammatory response. Here we identify DNA-dependent protein kinase (DNA-PK) as a DNA sensor that activates innate immunity. We ...