To carry out real-time PCR, researchers have to choose not only what primers to design (see Box 2) but also what detection chemistry to use. In many cases these decisions will be influenced ...

A method for genetically barcoding Drosophila is developed and used to tag defined cell populations in vivo for single-cell transcriptomics experiments and to enable multiplexed behavioral analysis.

North Carolina State University researchers continue to push the field of plant disease diagnosis forward, developing ...

This study provides a valuable new resource to investigate the molecular basis of the particular features characterizing the pipefish embryo. The authors found both unique and shared gene expression ...

North Carolina State University researchers continue to push the field of plant disease diagnosis forward, developing ...

It chooses two amino acids nearby in linear sequence, so only one mutagenic primer is needed ... single-substitution approach using error-prone PCR increased it only to 7.4. Both approaches ...

Multiplex: the 11 amplicons were amplified with two reactions mixtures (six odd-numbered primer pairs and five even-numbered prime pairs) and then pooled. The schematic figure was drawn with Biorender ...

Primers were designed and developed for analysing Desulfovibrio populations in the bowel using real time polymerase chain reaction (PCR). Conclusions: Real time PCR analysis of desulfovibrios was an ...

cDNA was synthesised from 1 × 10 −5 to 1 μg of total RNA with reverse transcriptase (BRL, Gaithersburg, Maryland, USA) using random primers (Takara). Subsequently, the reverse transcriptase reaction ...

PCR amplification of exon V generated a 97 bp fragment (fig ... The S allele was resistant to digestion producing a fragment of 133 bp that had been shortened at the primer control digestion site ...