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To generate mosaic transgenic fish lines, a 1 nL mixture of plasmid DNA (40 ng/μL ... R3189S) for 1 h. The linearized product was purified according to manufacturer specifications via gel selection ...

The cDNAs were subjected to polymerase chain reaction (PCR) amplification and cloned into the pCAGGS vector. The plasmid was linearized using SalI and BamHI (NEB, Ipswich, MA, USA) for microinjection ...

Department of Biomedical Engineering, University of California, Irvine, California 92617, United States Center for Synthetic Biology, University of California, Irvine, California 92617, United States ...

This novel passivity-based bilateral teleoperation using linear MPC and linearized energy tank reduces the computational effort of existing passive non-linear MPC controllers.

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. This study shows that RNA decay rather than ...

Preparation of Plasmid and mtDNA Mixtures for Method Validation Isolated pcDNA3.1 plasmid or mtDNA, extracted from isolated mitochondria, were diluted in triplicates and quantified separately using ...

Many unicellular green algae can store carbon in the form of starch under adverse environmental conditions, such as high light and nutrient starvation (Ball et al., 1990). Microalgae are considered as ...