First, employing the native regulatory element responsible for governing the expression of the target gene ensures that the expression pattern of the GFP reporter closely mirrors that of the gene of ...

An inadequate supply of amino acids leads to accumulation of uncharged tRNAs, which can bind and activate GCN2 kinase to reduce translation. Here, we show that glutamine-specific tRNAs selectively ...

1 month after LV PGK-GFP was injected to label NSCs in the DG, a unilateral transection of the PP was performed. BrdU was injected for 3 days and the brain was analyzed 4 days later. Although these ...