Wu and colleagues confirmed that cultured-microglia-carrying lipid droplets struggled with phagocytosis, poorly engulfing ...

This will allow quicker and easier gene knock-down experiments, for example, because having on-board Cas9 makes more room in vectors used to deliver the guide RNA to contain multiple instructions for ...

Knockout mice are used to study what happens in an organism when a particular gene is absent. Studying knockout mice can provide information about how the knocked-out gene normally functions ...

It’s a new way to create “bi-paternal” mice that can survive to adulthood—but human applications are still a long way off.

Knock-out mice carry a gene that has been inactivated, which creates less expression and loss of function; knock-in mice are produced by inserting a transgene into an exact location where it is ...

"We generated Tle6 hetero knockout mice to investigate the effects of Tle6 deficiency in male mice. We performed genome editing of the embryos using the CRISPR-Cas9 system and electroporation to ...

To get a better sense of the importance of the structural differences, the team then created Ribosome ST knockout mice. In the males, core ribosomes were unable to pick up the slack—and as a result, ...

Myocardial fibrosis is a key process for heart failure development that may lead to severe phenotypes, including cardiac ...

Mouse models for hemophilia A are commonly generated by factor VIII (FVIII) knockout, however, these mice rapidly develop anti-FVIII antibodies during repetitive FVIII administration. To overcome this ...

Knocking out the activity of a gene provides valuable clues about what that gene normally does. However, creating and characterizing mice with gene knockouts is a time-consuming and difficult process, ...