'Flycode' system allows simultaneous analysis of 25 antibodies in mice to reduce animal testing
Many modern drugs are based on antibodies. These proteins very specifically identify a certain structure on the surface of ...
eLife15d
Mitochondrial adenine base editing of mouse somatic tissues via adeno-associated viral delivery
The authors have demonstrated the use of adenine base editors delivered via adeno-associated viruses to introduce edits in the mitochondrial genome. The manuscript describes the methodology well, and ...
Frontiers3mon
Limitations of a proper SFTSV mouse model using human C-type lectin receptors
BJAB cells were infected with GFP-expressing SFTSV at a multiplicity ... SOTO-1). Secondary antibodies, Alexa594 conjugated anti-rabbit IgG or Alexa594 conjugated anti-mouse IgG (Invitrogen), were ...
The Scientist1y
Serendipity, Happenstance, and Luck: The Making of a Molecular Tool
GFP remained just an interesting luminescent protein found in some species of marine animals. In 1989, scientists studied genes of interest in cells using newly developed technologies. “But in all of ...
The Scientist1y
Spectral Flow Cytometry: Separating the Fluorophore Rainbow
To construct our panel, Kenneth Adusei, the lead author of the paper, and I performed a product search of the various rat antibodies that were available off-the-shelf. We created a reagent spreadsheet ...
eLife1y
Regulation of different phases of AMPA receptor intracellular transport by 4.1N and SAP97
Quantification of the GFP staining revealed an almost complete disappearance ... and transferred in PBS/BSA (1%). Secondary antibody anti-mouse Alexa-fluo588 or anti-rabbit Alexa-fluo488 was applied ...
Frontiers2y
Evaluating the transduction efficiency of systemically delivered AAV vectors in the rat nervous system
The viruses used were PHP.S-CAG-tdTomato (59462-PHP.S), AAV9-CAG-tdTomato (59462-AAV9), and AAVretro-CAG-GFP (37825-AAVrg). 2.1 × 10 11 vg/rat were injected intraperitoneally ... and blocked for ...