Eye and plasmid depictions were ... per manufacturer instructions. Briefly, HEK293 cells were transfected with NRL-Xpress or RNase H1 constructs (GFP-HR and GFP-dHR). Cells were washed with PBS 48 hr ...

Human embryonic kidney fibroblast (HEK-293) cells, were purchased from the ATCC ... As positive control, cells were transfected with a plasmid expressing GFP under the control of the strong and ...

Colorectal tumors harbor increased levels of phosphorylated eIF2α. Disrupting the eIF2Bα homodimer is detrimental for CRC cells. The ability to sense eIF2α phosphorylation is essential for CRC cell ...

In this report, we show, for the first time, that this dual PTM occurs in cells on mono-ADP-ribosylated (MARylated) PARP10 on Glu/Asp sites to form a MAR ubiquitin ester. We call this process mono-ADP ...

This requires the ability to accurately track cells, observing their function, movement and interactions in vitro and in vivo. GFP ioMicroglia is built from the company’s well-established ioMicroglia.

School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P.R. China School of Chemical & Material Engineering, Jiangnan University, Wuxi 214122, P.R. China ...

The membrane was incubated overnight at 4°C with primary antibodies specific for green fluorescent protein (GFP ... we co-transfected the pCAGGS-p72-HA plasmid and these 13 host protein plasmids into ...

HEK-293 cells stably expressing either the signal-connector or the negative control were selected after transfection of the pGPU6/GFP/Puro vectors. HEK 293/T24 cells co-expressing multiple ...