This useful study presents findings on the developmental roles of Nup107, a key nucleoporin, in regulating the larval-to-pupal transition in Drosophila melanogaster through its involvement in ecdysone ...

PCR-based techniques are methods that rely on the polymerase chain reaction (PCR) to amplify stretches of DNA by creating many identical or near-identical copies. For example, PCR amplification ...

This manuscript describes an important finding of the transcriptional control of a chimeric gene transfer agents (GTA) cluster in Bartonella by a processive anti-termination factor (BrrG). The ...

If Chalfie and Euskirchen wanted only the GFP DNA, they could amplify just the GFP gene by PCR, but that risked introducing mutations since PCR was error-prone at the time. Alternatively, they could ...

The PCR products and Myc tag plasmid were ... KOD-Plus-Mutagenesis Kit (Toyobo) was used to make a synonymous mutation of FBXO34 plasmid. CCNB1-GFP plasmid, MAP4-eGFP plasmid, and H2B-mCherry plasmid ...

Free-floating Fezf2-Gfp, CTB647 labeled sections were blocked for 1 h at room ... The resulting first strand cDNA was purified using AMPure XP beads before PCR amplification. The cDNA was added to a ...

Here we propose a new green fluorescent protein (GFP)-based method for T4 phage molecular imaging in living systems. The method employs decoration of a phage capsid with GFP fused to the N-terminus of ...