To date, we have cloned 110 eukaryotic transport proteins into a GFP-fusion vector: 30 from yeast and 80 from higher eukaryotic organisms. After obtaining colonies (Steps 2 and 3), ...

To generate the GFP fusion protein, insert the corresponding cDNA into the multiple cloning site of a pEGFP or other appropriate fluorescent protein–coding vector, joining the GFP sequence at ...

GFP is a common fusion protein tag that enables protein expression to be monitored, quantified, and localized within the complex environment of the cell (Lippincott-Schwarz 2001).

Drs. Martin Chalfie, Roger Y. Tsien, and Osamu Shimomura were the recipients. The 2008 Nobel Prize in chemistry went to three researchers who discovered the fluorescent protein GFP in jellyfish ...