Figure 1: Confocal imaging of LLC-PK-1 cells in mitosis. To generate the GFP fusion protein, insert the corresponding cDNA into the multiple cloning site of a pEGFP or other appropriate ...

and a cleavable GFP-Gag chimeric protein as a fluid-phase marker present inside the virion was developed to study HIV-1 entry (Miyauchi et al., 2009). This platform provided evidence that frequently ...

The research team began developing a workaround by creating a fusion protein based on the complex formed by BTLA and HVEM. The increased stability from combining these proteins allowed the group ...

The Food and Drug Administration has approved more than 100 monoclonal antibodies to treat a range of diseases. Other ...

To test this approach, we inserted a green fluorescent protein (GFP) nanobody (GFPnb) into the VP1 protein of AAV-DJ inside the capsid and replaced the AAV payload plasmid with a GFP expression ...

4c01781. Number values for repeat motif coverage and accession numbers of structural proteins (Table S1), amino acid composition of structural proteins (Tables S2–S4), extensional mechanical ...

Our data further show that HDAC6 deacetylates enkurin domain-containing protein 1 (ENKD1) at lysine 98 and thereby impedes its interaction with γ-tubulin, restraining the centrosomal localization of ...

Video S1. Scan through a confocal image stack in Z-axis showing the fluorescence of the GFP (green) and Cherry (red) fusion proteins in relation to the chloroplast autofluorescence (in blue). Video S2 ...

The experiment was conducted using MO.Control v1.6.1 and data analysis was performed using MO.Affinity Analysis v2.3. By introducing a fluorescent label GFP to the FgDHODHII protein while maintaining ...

To address these challenges, this paper proposes a novel Automatic Matching Method for Bone Stick Fragments that integrates Siamese networks with Sequence Similarity Metric Algorithms. Our method ...

MagIC technique still has certain limitations resulting from formaldehyde fixation, use of bacterial-expressed recombinant H1.8-GFP, and potential effects of magnetic beads and/or spacer on protein ...