This study provides compelling evidence that kinesin's stepping mechanism is governed by strain-induced conformational changes in its nucleotide-binding pockets. Using pre-steady state kinetics and ...
Department of Chemistry, Faculty of Arts and Sciences, Center for Advanced Materials Research, Beijing Normal University, Zhuhai 519087, China Key Laboratory of Theoretical and Computational ...
From drug discovery to protein research, the MST assay is revolutionizing how scientists analyze ... The technique is label-free or fluorescence-based, depending on the user's needs and the type of ...
Fluorescent Detection Compared to Other Methods Outside of fluorescence, colorimetry is another method of detecting target compounds or cells within a sample. In colorimetric assays, the target ...
After photographing, the GFP fluorescence intensity of HAdV-GFP in cells was analyzed ... Cytotoxicity testing was performed using the CellTiter Glo luminescent cell viability assay kit (Promega) to ...
The calcein AM assay was based on the live and dead assay protocol ... were selected for labeling to avoid overlapping of fluorescent spectra during imaging. The gene of green fluorescent protein EGFP ...
After permeabilization, coverslips were washed twice with Digitonin Assay Buffer and processed for subsequent ... Half-cilium FRAP was performed as described previously (Hu et al., 2010). The GFP ...
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