The GFP expression constructs were transiently transfected into human kidney 293T or COS-7 cells 7. The amount of GFP protein (P) produced was quantified by western blot using rabbit anti-GFP ...
Poor expression of fusions. Check for expression of GFP fusions on SDS-PAGE. Vary concentrations of inducer to obtain roughly stoichiometric levels of expression. We find that 0–20 μM IPTG and ...
“I proposed to use GFP as a reporter for gene expression in bacteria ... San Diego made possibly the most striking contributions. With a background in dye chemistry, Tsien saw the value a fluorescent ...
Results that may be inaccessible to you are currently showing.
Hide inaccessible results
Feedback