The GFP expression constructs were transiently transfected into human kidney 293T or COS-7 cells 7. The amount of GFP protein (P) produced was quantified by western blot using rabbit anti-GFP ...
Poor expression of fusions. Check for expression of GFP fusions on SDS-PAGE. Vary concentrations of inducer to obtain roughly stoichiometric levels of expression. We find that 0–20 μM IPTG and ...
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