In particular, GFP represented a considerable step forward ... “The output is basically the three-dimensional distribution of fluorescence in vivo,” says Yared. “What you get is a fully ...

Fluorescent proteins have come along way since the discovery of GFP from the jellyfish Aequorea victoria in the early 1960s. Today, both natural and engineered fluorescent proteins come in a ...

elegans worm, zebrafish, and mice, they confirmed that the reporter also tracked glutamate in vivo, a finding that ... they swapped the type of GFP protein, producing a more photostable reporter with ...

Taking a risk on error-prone PCR, Euskirchen amplified only the GFP coding sequence and inserted it into an expression vector to transform back into E. coli. When Euskirchen imaged her bacteria using ...