This study compiles a versatile toolbox of different technologies that allow robust characterization of mRNA IRESes in cells and embryonic tissues. Circular RNA (circRNA) split-EGFP reporters confirm ...

One-cell-stage zebrafish embryos were injected with 1-2 nL of capped eGFP-nanos3’UTR mRNA using a pico-liter injector (Warner Instruments, PLI90A) and needles pulled from glass capillary tubing ...

The study protocol was approved by the Veterans Affairs Medical Center's research and development committee. There was no direct contact with human subjects in this study, therefore the hospital's ...

there are technologies available to help analyze the complex LNP systems used in delivering mRNA, such as the ultra-high-performance liquid chromatography-charged aerosol detector (UHPLC-CAD ...