For transcription, a solution containing 2 μM of the double-stranded DNA, 6.25 mM rNTP, 80 mM HEPES-KOH pH 7.5, 24 mM MgCl 2, 2 mM spermidine, 40 mM DTT, 20 μg/mL pyrophosphatase (Roche) and a ...

Four endogenously expressed genes are assayed together with RNA and DNA spikes in the samples ... Formation of PCR products of expected length was confirmed by agarose gel electrophoresis. Cycle of ...

We ligated adapters directly to both ends of the small RNA, followed by reverse transcription to synthesize cDNA. After PCR amplification, we separated the target DNA fragments using PAGE gel ...

This study shows that RNA decay rather than the presence of premature termination codons triggers a TA-like response.

Download the CBSE Class 12 Biology Sample Paper 2025 curated by experts for last-minute revision. Based on past exams and key ...

While human skin shares gel-like characteristics, it has unique qualities that are very hard to replicate. It combines high stiffness with flexibility, and it has remarkable self-healing ...

That’s why when you mix sea moss with water, it forms a gel. Gel is just one form in which sea moss can be consumed: It’s also commonly available in capsules or raw (one of my experts said the ...

Using this approach, we investigate the long-term behavior of Ni-rich NMC cells and examine ways to prevent degradation. This work also showcases the incredible durability of single-crystal cathodes, ...

Then, with the help of your doctor or consultant physiotherapist, and depending on the type and scale of your injury, you might be recommended an anti-inflammatory gel to use for a limited period ...