can compete with free hormone for a limited number of antibody-binding sites in a classical competitive immunoassay format. Though conceptually attractive, this approach is technically difficult ...

HTRF Transcreener ADP assay is a competitive immunoassay performed in two steps, the enzymatic step followed by the detection step (Fig. 1a). Standard curves are generated to mimic ADP generation ...

Here we describe the HTRF kits for cAMP detection. All Cisbio cAMP kits are based on a competitive immunoassay between native cAMP produced by cells and cAMP labeled with the acceptor dye d2.