During cell therapy development, some scientists are moving away from viral transfection and toward electroporation for introducing CRISPR systems and other nucleic acid payloads. As electroporation ...

In this article, we discuss transformation, bacterial transformation in particular, and how scientists can utilize it to ...

Electroporation works well with cell lines that are refractive to other techniques, such as calcium phosphate–DNA coprecipitation. But as with other transfection methods, the optimal conditions ...

Electroporation uses short electric pulses to create transient pores in a cell’s membrane, allowing researchers ... using the Neon NxT system that researchers can follow for optimal transfection ...

1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...

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