If desired, subclone the DNA fragment encoding the GFP fusion protein into the pIRES vector. The bicistronic expression vector pIRES can be used to generate stable cell lines expressing GFP-tagged ...

This study provides a potentially useful investigation into the positive role of BDNF/TrkB signaling in implanted dental pulp stem cells to enhance dentin regeneration in the context of dental caries.

Researchers successfully use a nonviral, piggyBac transposon system to introduce a transgene into cynomolgus monkeys.

Japanese researchers used a nonviral piggyBac system to genetically modify cynomolgus monkeys, enabling more accurate disease ...

Researchers in Japan successfully used a nonviral system to introduce a transgene – that is, a gene that has been ...

Genetic engineering in non-human primates has long been limited by the need for virus-based gene delivery methods. Recently, ...

Red fluorescent protein was localized to cell membranes, and green fluorescent protein was localized to cell nuclei. Expression was confirmed across all tissues examined, including germ cells ...

RNAi – functions specifically to silence, or deactivate, genes. Among other applications, it promises to be groundbreaking as ...

(A) Relative gene expression levels of chaperones and co-chaperones in cells expressing ER- FlucDM-eGFP ... Within 3 minutes, eGFP fluorescence intensity was measured by selecting the GFP channel of ...

Cell-free systems, which can express an easily detectable protein with a DNA or mRNA template without constraints of living ...