If desired, subclone the DNA fragment encoding the GFP fusion protein into the pIRES vector. The bicistronic expression vector pIRES can be used to generate stable cell lines expressing GFP-tagged ...

This requires the ability to accurately track cells, observing their function, movement and interactions in vitro and in vivo. GFP ioMicroglia is built from the company’s well-established ioMicroglia.

After erylysis, cells labeled with Mac-1-Pe, B220-Pe, Gr-1-Pe and CD4-Pe (Pharmingen, Heidelberg, Germany), were analyzed for GFP-expression. Dead cells (propidium iodide-positive) were excluded.

ClonTech optimized GFP for expression in mammalian cells, licensed, and commercialized as enhanced GFP, or eGFP in 1996. While many groups worked with GFP to change its stability or activity, Roger ...

Mapping out gene expression is informative ... to other imaging-based techniques and successfully detected green fluorescent protein (GFP) mRNA in expected cell types and regions of the root tip of an ...

Time-lapse confocal live imaging of 8-week K14:H2B-GFP murine MG explant in which all MG cells are GFP+ ... the researchers analyzed gene expression in normal human eyelid samples and in human ...