LCGC International interviewed Bob Pirok from the University of Amsterdam, Netherlands to discuss strategies for enhancing ...

Courtesy of Pierce Biotechnology Though well traveled, the road to two-dimensional gel analysis can be slow going. Pierce Biotechnology in Rockford, Ill., has looked to the past in developing four new ...

When performing 1D and 2D gel electrophoresis within your laboratory, you doubtlessly pay a great deal of attention to your experimental design, sample preparation, and technical execution, likely ...

Depending on the preceding workflow, the enzymatic digestion of proteins is performed either in-gel or in-solution. Generally, the in-gel digestion methodology has become routine for proteins ...

Two-dimensional polyacrylamide gel electrophoresis, or 2D PAGE, was introduced in 1975 concurrently by Joachim Klose 7 and Patrick H. O’Farrel. 8 In the first dimension, proteins are separated ...

Figure 1. One dimensional SDS polyacrylamide gel electrophoresis using the UVP GelSolo. Mouse serum was run on the gel in 1:2 dilution series from 20 μg to 39 ng on a 4 - 12 % Bolt Bis-Tris Plus ...

Three methods for separation of complex protein or peptide samples are preferred in proteomics: denaturing polyacrylamide gel electrophoresis (PAGE) or sodium dodecyl sulfate polyacrylamide gel ...