if the various PCR products can be size-fractionated sufficiently. Even old, degraded DNA yields enough signal to perform the analysis. For maximum signal strength I have optimized the protocol ...
When using this protocol, emulsification should be performed ... Pipet the emulsion into the wells of a PCR plate as 10 aliquots of 50 μl. Pipet 50 μl of the aqueous phase (Step 3) into a ...
The Applied Biosystems ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems thermal cyclers with the flexible configuration and control features ...
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