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We used the primers noted in Table 1. Standard curves were generated from known quantities of each target gene with linearized plasmid DNA. We used 10 times dilution series for each known target, ...

To generate mosaic transgenic fish lines, a 1 nL mixture of plasmid DNA (40 ng/μL ... R3189S) for 1 h. The linearized product was purified according to manufacturer specifications via gel selection ...

This paper presents the important finding that BNIP3/NIX, a mitophagy receptor, and its binding to ATG18 are required for mitophagy during muscle cell reorganization in Drosophila. Although the ...

Department of Biomedical Engineering, University of California, Irvine, California 92617, United States Center for Synthetic Biology, University of California, Irvine, California 92617, United States ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. This study shows that RNA decay rather than ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Widespread control of gene expression through ...

Preparation of Plasmid and mtDNA Mixtures for Method Validation Isolated pcDNA3.1 plasmid or mtDNA, extracted from isolated mitochondria, were diluted in triplicates and quantified separately using ...

These will contain the experimental results where the first index and last index are where an R-loop has been experimentally found inside the given plasmid. If you would like to reproduce the model ...