The Maestro In-Vivo Imaging System from Cambridge ... in this system, the GFP chromophore is only capable of maturation and fluorescence after the assembly of two independently soluble and stable ...

This requires the ability to accurately track cells, observing their function, movement and interactions in vitro and in vivo ... traditional imaging methods where cells are fixed before staining.

Taking a risk on error-prone PCR, Euskirchen amplified only the GFP coding sequence and inserted it into an expression vector to transform back into E. coli. When Euskirchen imaged her bacteria using ...