and it produced GFP variants with different colours and capacities for changing colour in response to environmental stimuli. With the cloning of new fluorescent proteins from species other than ...

Lukyanov, of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, and colleagues report the isolation of a GFP variant called KillerRed that acts as a ...

Thus, fused polypeptides may help producing GFP variants with a more robust folding, e.g. when poorly folding spectral variants are used or when fusion to insoluble proteins is needed. The GFP ...

With only a small amount of experimental data (roughly 1,000 GFP variants), the model produced a “fitness landscape,” a three ...

The GFP reporting system was then used to test the specificity of these variants. The variant ChCas12b-D496A showed less off-target activity according to GUIDE-seq analysis, which might make it a ...