The cells were incubated with the primary antibody [5 μg/200,000 cells, IgG rabbit anti-rat von Willebrand factor (vWF) polyclonal, No. ab 6994; Abcam, Cambridge, England] at 20°C for 30 min.
This study provides a potentially useful investigation into the positive role of BDNF/TrkB signaling in implanted dental pulp stem cells to enhance dentin regeneration in the context of dental caries.
The viruses used were PHP.S-CAG-tdTomato (59462-PHP.S), AAV9-CAG-tdTomato (59462-AAV9), and AAVretro-CAG-GFP (37825-AAVrg). 2.1 × 10 11 vg/rat were injected intraperitoneally ... and blocked for ...
Mouse anti-His tag mAb HRP conjugate (Sino Biological ... Primary antibodies for immunodetection were sourced as follows: Flag antibody (CST, 2368S) and GFP antibody (CST, 2956S).