The GFP expression constructs were transiently ... advantage over mutagenesis methods of maintaining intact the protein structure under study.

Shimomura attempted to isolate this mystery protein under various conditions ... However, nobody knew if this would be possible. GFP had a cyclized portion in its structure, and many people thought ...

This process has yielded optimized versions of many important proteins, including green fluorescent protein (GFP ... from DNA ...

The crystal structure of GFP, solved by James Remington ... colours exhibited by some corals resulted from fluorescent proteins like GFP. No one had previously thought of this because fluorescence ...