Eye and plasmid depictions were ... per manufacturer instructions. Briefly, HEK293 cells were transfected with NRL-Xpress or RNase H1 constructs (GFP-HR and GFP-dHR). Cells were washed with PBS 48 hr ...
Human embryonic kidney fibroblast (HEK-293) cells, were purchased from the ATCC ... As positive control, cells were transfected with a plasmid expressing GFP under the control of the strong and ...
This requires the ability to accurately track cells, observing their function, movement and interactions in vitro and in vivo. GFP ioMicroglia is built from the company’s well-established ioMicroglia.
This study aimed to encapsulate plasmid DNA encoding the TP53 tumor suppressor gene (pEGFP-N1-TP53) within zeolitic imidazolate framework-8 (ZIF-8) MOFs and ZIF-8-PEI. Subsequently, the transfection ...
In this report, we show, for the first time, that this dual PTM occurs in cells on mono-ADP-ribosylated (MARylated) PARP10 on Glu/Asp sites to form a MAR ubiquitin ester. We call this process mono-ADP ...
Colorectal tumors harbor increased levels of phosphorylated eIF2α. Disrupting the eIF2Bα homodimer is detrimental for CRC cells. The ability to sense eIF2α phosphorylation is essential for CRC cell ...
Article subjects are automatically applied from the ACS Subject Taxonomy and describe the scientific concepts and themes of the article. In the preceding few decades, the emergence of RNA-based ...