Transgenic animals continue to play an essential role in many aspects of zebrafish research, including the development of disease models. The most widely used system for zebrafish transgenesis is the ...

Polyphosphate (polyP) exists in all life forms; however, its biological functions in metazoans are understudied. Here, we explored Drosophila as the first genetic model to explore polyP biology in ...

Subsequently, the samples underwent centrifugation twice at 10000g for 1 h at 4 °C to eliminate excess Nile Red molecules, after which the NR-labeled exosomes were resuspended in a PBS solution. To ...

Department of Anatomy and Neurobiology, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea Department of Precision Medicine, Graduate School, Kyung Hee University, Seoul 02447, ...

These antimicrobial peptides are considered vital components of the body's first line of defense as they attack and kill bacteria. Experiments showed that human cells with active proteasomes ...

Transient expression of outer envelope proteins or protein domains such as CHUP1-TM–GFP, outer envelope protein of 7 kDa–GFP, or outer envelope protein of 24 kDa–GFP at high levels led to the ...

The following fluorescence settings were used for detection: DAPI: 419–442 nm, GFP: 498–563 nm, Alexa 555: 562–598 nm. Recording was performed sequentially to avoid bleed-through using a conventional ...

Combined, these findings show that CMA displays a broad targetability on multiple steps of the EMT process and indicate a substantial role of CMA in regulating the stability of pro-metastatic and ...