If desired, subclone the DNA fragment encoding the GFP fusion protein into the pIRES vector. The bicistronic expression vector pIRES can be used to generate stable cell lines expressing GFP-tagged ...

e.g. when poorly folding spectral variants are used or when fusion to insoluble proteins is needed. The GFP expression constructs were transiently transfected into human kidney 293T or COS-7 cells 7.