If desired, subclone the DNA fragment encoding the GFP fusion protein into the pIRES vector. The bicistronic expression vector pIRES can be used to generate stable cell lines expressing GFP-tagged ...

Poor expression of fusions. Check for expression of GFP ... prey vector orientation. We have successfully used spacers up to 15 amino acids in length. Bait or prey misfolded. The analyte protein ...

However, the duo did not purify any luciferin-related proteins from the jellyfish ... Euskirchen amplified only the GFP coding sequence and inserted it into an expression vector to transform back into ...