This study provides compelling evidence that kinesin's stepping mechanism is governed by strain-induced conformational changes in its nucleotide-binding pockets. Using pre-steady state kinetics and ...
Department of Chemistry, Faculty of Arts and Sciences, Center for Advanced Materials Research, Beijing Normal University, Zhuhai 519087, China Key Laboratory of Theoretical and Computational ...
Department of Medicinal Chemistry, Ernest Mario School of Pharmacy, Rutgers, the State University of New Jersey, Piscataway, New Jersey 08854, United States ...
while the second can be dedicated to special applications such as side population assay, basic GFP sorting or optimized for multiple fluorescent protein analysis and sorting. The integrated ...
The calcein AM assay was based on the live and dead assay protocol ... were selected for labeling to avoid overlapping of fluorescent spectra during imaging. The gene of green fluorescent protein EGFP ...
Appropriate signal compensation was set using single color and fluorescent minus one ... Promega Dual-Luciferase Reporter Assay System (#E1910). Adipocytes from mice that underwent BMT from transgenic ...
Site-specific fluorescent labeling of proteins inside live mammalian cells has been achieved by employing Streptolysin O, a bacterial toxin which forms temporary pores in the membrane and allows ...
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