Fluorescent proteins have come along way since the discovery of GFP from the jellyfish Aequorea victoria in the early 1960s. Today, both natural and engineered fluorescent proteins come in a ...
Taking a risk on error-prone PCR, Euskirchen amplified only the GFP coding sequence and inserted it into an expression vector to transform back into E. coli. When Euskirchen imaged her bacteria using ...