Determine the level of expression of the GFP fusion protein in ... the plate during the steps after Step 6; thus, care must be taken to avoid killing the cells by drying (with practice, about ...
Sorted populations were reanalyzed for GFP expression and routinely demonstrated purities ranging from 90 to 95%. Retroviral transduction and transplantation of rhesus macaque hematopoietic cells.
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