We ligated adapters directly to both ends of the small RNA, followed by reverse transcription to synthesize cDNA. After PCR amplification, we separated the target DNA fragments using PAGE gel ...

The other fragment was snap frozen in liquid nitrogen for protein isolation and western blot analysis or placed in RNAse free tubes containing 500 μl of RNA Later Solution ... dodecyl ...

2.7 RNA Isolation, Reverse Transcription ... The cell extracts were separated by 4%–12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and equal amounts of protein (70 μg ...

This study shows that RNA decay rather than the presence of premature termination codons triggers a TA-like response.

FAMU-FSU College of Engineering researchers have created a new method for studying protein degradation within immune cells ...

A new study conducted by researchers at Hokkaido University has unveiled a novel class of zwitterionic phospholipids capable ...

Targeted protein degradation is a therapeutic strategy that utilizes the cell’s natural machinery to eliminate disease-causing proteins. The field traces its origins to the development of ...

Download the CBSE Class 12 Biology Sample Paper 2025 curated by experts for last-minute revision. Based on past exams and key ...

Polyphosphate (polyP) exists in all life forms; however, its biological functions in metazoans are understudied. Here, we explored Drosophila as the first genetic model to explore polyP biology in ...