If desired, subclone the DNA fragment encoding the GFP fusion protein into the pIRES vector. The bicistronic expression vector pIRES can be used to generate stable cell lines expressing GFP-tagged ...

A growing body of work suggests that cell metabolism — the chemical reactions that provide energy and building materials — ...

In the rapidly evolving field of drug discovery, single-cell analysis has become an invaluable tool for understanding cellular heterogeneity and molecular | Drug Discovery And Development ...

This important study provides solid evidence that glucosylceramide synthase (GlcT), a rate-limiting enzyme for glycosphingolipid (GSL) production, plays a role in the differentiation of intestinal ...

Transgenic animals continue to play an essential role in many aspects of zebrafish research, including the development of disease models. The most widely used system for zebrafish transgenesis is the ...

Only recently has the ribosome - one of the oldest molecular machines in evolutionary terms - been recognized as an active regulator of gene expression at the level of protein biosynthesis.

the other carrying a different resistance gene and GFP in the chloroplast genome. To facilitate genetic exchange between cells from the two different plants, they grafted one plant onto the other ...

In this study we evaluated changes in IL-17 expression in the inflamed mucosa and in the serum of patients with inflammatory bowel disease (IBD). Methods: Tissue samples were obtained endoscopically ...