and expression testing of vectors featuring various affinity tags (i.e., His, GST, FLAG) and/or fusion partners (i.e., TRX, MBP, EGFP) in order to maximize the expression level of the target protein ...

The major core services are the following: Up-front consultation on experimental design and assistance with construction of various recombinant protein expression vectors. Expression of recombinant ...

As an agent, your first responsibility is to identify the league for which your particular client (protein) is best suited ... Beginning with this issue, we will take a look at expression vectors ...

Figure 1: GFP-fragment reassembly vectors and cloning linker sequences ... Avoid fusions to the GFP fragments at the site of protein-protein interaction and consider optimizing the spacer length ...

To compare cell growth, protein expression level and final purified target yield, we also performed a standard induction with IPTG using the same vectors, host and fusion proteins. The crude ...

In the final step, they pick resistant colonies to isolate the plasmids, which are then sequenced and cloned into the desired protein production vectors for various applications.

If the treatment is successful, the new gene will make a functional protein. (Illustration ... and non-dividing cells and provide high levels of gene expression. However, adenoviral vectors can ...