Early efforts to engineer red-shifted Aequeorea victoria GFP (avGFP) variants led to the development of enhanced GFP (EGFP) and yellow fluorescent proteins with emission maxima at approximately ...

In 2004, they published a follow-up to a 2002 paper reporting the first monomeric red fluorescent protein, mRFP1, this time using molecular evolution to improve the protein's properties and create new ...