Individual recombinant viral isolates are plucked from agarose and used to infect Sf9 insect cells. The infected Sf9 cells (for intracellular proteins) or culture supernatant (for secreted proteins) ...

This expression vector was constructed in accordance ... We transiently produced a recombinant protein as proof of concept. Factor IX is a secreted glycoprotein used in hemophilia B treatment.

The major core services are the following: Up-front consultation on experimental design and assistance with construction of various recombinant protein expression vectors. Expression of recombinant ...

Transfect the cells with the recombinant vector using Lipofectamine 2000 ... on the coverslip to check the level of GFP fusion protein expression with high-resolution fluorescence microscopy ...

capitalizing on the CFTR protein expression and purification expertise of their laboratory to offer recombinant CFTR proteins and cell/membrane biomass. Up to milligram quantities of analytically pure ...

In the early and mid-1980s, scientists used phages to express recombinant ... proteins of interest into plasmids that carry a phage coat protein gene, an antibiotic resistance gene, and a packaging ...

making plant cell-based bioproduction a transformative platform for producing high-value recombinant proteins. “Implementing these strategies would significantly improve the efficiency of plant ...