and expression testing of vectors featuring various affinity tags (i.e., His, GST, FLAG) and/or fusion partners (i.e., TRX, MBP, EGFP) in order to maximize the expression level of the target protein ...

The major core services are the following: Up-front consultation on experimental design and assistance with construction of various recombinant protein expression vectors. Expression of recombinant ...

As an agent, your first responsibility is to identify the league for which your particular client (protein) is best suited ... Beginning with this issue, we will take a look at expression vectors ...

Figure 1: GFP-fragment reassembly vectors and cloning linker sequences ... Avoid fusions to the GFP fragments at the site of protein-protein interaction and consider optimizing the spacer length ...

In the final step, they pick resistant colonies to isolate the plasmids, which are then sequenced and cloned into the desired protein production vectors for various applications.

If the treatment is successful, the new gene will make a functional protein. (Illustration ... and non-dividing cells and provide high levels of gene expression. However, adenoviral vectors can ...

But the protein kinases that suppress ... and conducted further experiments inducing the expression of functionally modified enzymes using viral vectors. Consequently, they found that PKA ...

Viral vectors expressing proneural genes can stimulate adult mammalian retinal glia to become immature neurons. These glia-derived neurons share properties with the bipolar, amacrine and retinal ...

Figure 1: Fluoresence and phase-contrast microscopic evaluation of murine hematopoietic colonies for GFP expression ... stable high-titer retroviral vectors that carry a human γ-globin gene ...