Scientists commonly do this using gel electrophoresis analysis, which can determine plasmid length and conformation based on how DNA migrates within a gel. The Agilent Fragment Analyzer system offers ...

from the plasmid DNA (=vector). Following separation of insert and vector by electrophoresis, you will elute the insert from the agarose. More background information about agarose gel electrophoresis ...

Prepare plasmid DNA using standard miniprep protocols ... Analyze the size and quality of the products by electrophoresis through an agarose gel. Select several colonies, grow each in 5 ml ...