While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

SpectraPhysics InSight X3 dual laser, including a 680-1300 nm tunable laser and a fixed 1045 nm laser.

A miniature head-mounted two-photon microscope small enough for a rat to ... One widely used method of in vivo fluorescence imaging in the brain is to remove or thin the region of the skull ...

In the last decade, two techniques have revolutionized fluorescence microscopy: the development of genetically encoded fluorophores, such as GFP 5,6 and the invention of two-photon microscopy 7.

The Movable Objective Microscope ® (MOM ®) is a two-photon microscope capable of imaging deep within living specimens when combined with a Ti:Sapphire Laser. The MOM design is unique in ...

The multiphoton techniques include: two-photon excited fluorescence ... Upright multiphoton microscope optimized for intravital imaging of anesthetized small laboratory animals. The system allows ...

The Leica SP8 is also a multi-photon microscope that can utilize the internal detectors from the visible confocal as well as two external NDD detectors for direct detection. The microscope is an ...

They show that the new method enables in vivo imaging of neuronal activity in a mouse cortex and can image at speeds ten times faster than traditional two-photon microscopy while also reducing the ...