The recent findings of Waldo et al. 1 demonstrate that the fusion of the green fluorescent protein (GFP) to insoluble proteins dramatically reduces its folding ability in prokaryotic cells.

Figure 1: Confocal imaging of LLC-PK-1 cells in mitosis. To generate the GFP fusion protein, insert the corresponding cDNA into the multiple cloning site of a pEGFP or other appropriate ...

Previous studies that investigated these events looked at isolated ribosomal proteins as opposed to the multiple ribosomes ... by using the intensity of GFP-tagged antibodies on the polypeptide to ...

The model was able to predict optimized GFP sequences that had as many as seven different amino acids from the protein ...