Early efforts to engineer red-shifted Aequeorea victoria GFP (avGFP) variants led to the ... regions for particular experiments. Although mCherry remains the optimum monomeric red fluorescent ...

The team found that its mOrange protein had a different chromophore structure from that of the green or red varieties. Another color, mCherry, turned out to be more photostable than expected.

This configuration is ideal for in vivo imaging of whole specimens. The Ultima IV is equipped with a tunable two-photon excitation laser that allows dual color imaging of CFP/GFP/YFP together with ...