A biotinylated anti-CD34 antibody from CellPro (Bothell, WA) was used for the immunoselection. Cells were transduced with a GFP-containing retroviral vector for 3.5 days on Retronectin- (Takara ...
Determine the level of expression of the GFP fusion protein in the selected clones by probing western blots of cell extracts with antibodies to the tagged protein and determining the percentage of ...
GFP remained just an interesting luminescent protein found in some species of marine animals. In 1989, scientists studied genes of interest in cells using newly developed technologies. “But in all of ...
Even with significant expansion in the global market for antibodies used in clinical care and research, scientists recognize that there is still untapped potential for finding new antibodies. Many ...
The Food and Drug Administration has approved more than 100 monoclonal antibodies to treat a range of diseases. Other ...
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Fusion proteins enable the creation of monoclonal antibodiesThe Food and Drug Administration has approved more than 100 monoclonal antibodies to treat a range of diseases. Other antibodies are used by physicians to diagnose conditions or by scientists to ...
The 2008 Nobel Prize in Chemistry will go to a trio of researchers who discovered, expressed, and developed green fluorescent protein (GFP) and revolutionized the way that biologists visualize living ...
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