If Chalfie and Euskirchen wanted only the GFP DNA, they could amplify just the GFP gene by PCR, but that risked introducing mutations since PCR was error-prone at the time. Alternatively, they could ...

Figure 1: GFP-fragment reassembly vectors and cloning ... which we prefer for libraries, we perform colony PCR with primers 5′-tagcggatcctacctgacgc-3′ and 5′-ttcgggctttgttagcagcc-3′.

Techniques such as PCR, GFP/FRET and RNAi have led to quantum leaps in experimental sophistication. These developments have resulted in a recasting of the science landscape, with an increased ...