To generate the GFP fusion protein, insert the corresponding cDNA into the multiple cloning site of a pEGFP or other appropriate fluorescent protein–coding vector, joining the GFP sequence at ...

Figure 1: GFP-fragment ... or swapping bait-prey vector orientation. We have successfully used spacers up to 15 amino acids in length. Bait or prey misfolded. The analyte protein most likely ...