The crystal structure of GFP, solved by James Remington and Roger Tsien, greatly helped this endeavour as it revealed the exact arrangement of the fluorophore. Mutagenesis of amino acids bound to ...

Besides GFP and two-photon ... microscopes that can systematically separate and individually track dozens of fluorophores has provided a multicolor experimental palette to cell biologists and ...

However, the number of markers that can be included in a flow cytometry panel is limited by the number of fluorophores with distinguishable emission spectra. Spectral flow cytometry, an evolved ...

The sequence of the RNA component, the aptamer, can be fused to the 3' end of the gene of interest, similar to the addition of a FLAG tag or GFP to a protein of interest ... a linker, and a ...